Evaluation Method of Hepatoprotective Effect of Health Food

Male SD rats or male BALB/c mice

1.  CCl4-induced liver damage model

2.  APAP-induced liver damage model

3.  TAA-induced liver fibrosis model

4.  Alcohol-induced fatty liver disease model

5.  High fat diet-induced nonalcoholic fatty liver disease model

Test items are different depending on the animal model:

1.  Plasma or serum: AST, ALT, TG, TC, etc

2.  Liver: glutathione, glutathione peroxidase, superoxide dismutase, catalase, TG, TC, glutathione reductase, glutathione S-transferase, collagen, liver fibrosis markers, etc

3.  Pathological examination

Evaluation Method of Blood Lipid Regulation Effect of Health Food

Male hamsters

1.  High fat diet-induced hyperlipidemia model (induction in advance)

2  .High fat diet-induced hyperlipidemia model (induction and treatment at the same time)

Serum TG, TC, LDL-C, HDL-C, fecal lipids, liver lipids, etc.

Evaluation Method of Immuno-modulatory Effect of Health Food

Male Balb/c mice

1.   Non-specific immune response

2.   OVA-specific immune response

Immune cell proliferation ability, natural killer cell activity, phagocytic cell activity, cytokine analysis, serum antibody analysis, cell surface marker analysis of spleen lymphocyte, etc.

Evaluation Method of Blood Glucose Regulation Effect of Health Food

Male db/db mice

spontaneously hyperglycemia db/db mice

Fasting and postprandial blood glucose, glucose tolerance test, fasting and postprandial plasma insulin, urine glucose, etc

Evaluation Method of Gastrointestinal Function Improvement Effect of Health Food

SD or Wistar rats

To evaluate whether the test article has one of the following four GI functions through different animal models:

1.   Improve digestion and absorption

2.   Improve intestinal microbiota

3.   Improve gastrointestinal motility

4.   Contribute to the protection of gastric mucosa

1.   Improve digestion and absorption: body weight, digestibility, enzyme activity

2.   Improve intestinal microbiota: Bifidobacterium, Clostridium perfringens

3.   Improve gastrointestinal motility: gastrointestinal motility (activated carbon), gastric mucosal protection, observation of the dry weight, grain number, moisture, and shape of feces

4.   Contribute to the protection of gastric mucosa:

     (1)   in vitro: survival rate of rat gastric mucosal cells

     (2)   in vivo: determination of gastric acid secretion in rats (Shay’s ulcer method)

Evaluation Method of Bone Health Protection Effect of Health Food

SD or Wistar rats

1.   Growing animal model

2.   Ovariectomized (OVX) animal model

Body weight, body length, femoral length or femoral plus tibia length, and at least 2 of the following 5 indexes: bone density, determination of trabecular bone, assessment of trabecular mineral apposition rate (MAR), biomechanical analysis, and metabolic biochemical indexes of osteoblast (OB) and osteoclast (OC).

Evaluation Method of Anti-fatigue Effect of Health Food (Exercise-induced Fatigue)

Healthy adults over 20 years old and have similar biological age

Randomized double-blind crossover study design

1.   Physical performance index: anaerobic exercise, maximum aerobic exercise, endurance, muscle strength, muscle endurance, muscle endurance strength, and power.

2.   Mechanism index: endocrine pressure index of physiological pressure, muscle tissue damage index, muscle tissue energy storage index.

Evaluation Method of Anti-aging Effect of Health Food

Animal models of senescence:

1.   aging rats or mice

2.   long-term experiment using young rats or mice

3.   senescence accelerate mouse (SAM)

4.   drug-induced aging animal:

      (1)   D-galactose induced senescence model

      (2)   Parkinson’s disease animal model (PD)

      (3)   Alzheimer’s disease animal model (AD)

Select an appropriate animal model of senescence for further experiment.

Test items are different depending on the animal model:

1.   Bioactive index of brain aging

2.   Bioactive index of liver, cardiovascular, blood, or kidney aging

3.   Anti-oxidant index

Evaluation Method of Body Fat Accumulation Reducing Effect of Health Food

Male SD rats

1.   High energy diet-induced obesity model (induction in advance)

2.   High energy diet-induced obesity model (induction and treatment at the same time)

Body weight, fat percentage, serum TC, free fatty acid, LDL-C, HDL-C, liver lipids, blood glucose, liver function index, kidney function index, etc.

Evaluation Method of Allergy Constitution Modulating Effect of Health Food

Balb/c or B6 mice

1.   Allergic model induced by intraperitoneal injection of allergen

2.   Respiratory allergy model

3.   allergic rhinitis model

serum antibody analysis, Immune cell proliferation ability, cytokine analysis, pulmonary function measurement or other allergy-related index, natural killer cell activity, phagocytic cell activity, etc.

Evaluation Method of Blood Pressure Regulating Effect of Health Food

Adults over 20 years old who have been diagnosed with higher blood pressure or mild hypertension by a physician and are not taking medication to control blood pressure

Double-blind, randomized, parallel-controlled

Blood pressure, safety monitoring