Evaluation Method of Bone Health Protection Effect of Health Food

SD or Wistar rats

1.   Growing animal model

2.   Ovariectomized (OVX) animal model

Body weight, body length, femoral length or femoral plus tibia length, and at least 2 of the following 5 indexes: bone density, determination of trabecular bone, assessment of trabecular mineral apposition rate (MAR), biomechanical analysis, and metabolic biochemical indexes of osteoblast (OB) and osteoclast (OC).

Evaluation Method of Anti-fatigue Effect of Health Food (Exercise-induced Fatigue)

Healthy adults over 20 years old and have similar biological age

Randomized double-blind crossover study design

1.   Physical performance index: anaerobic exercise, maximum aerobic exercise, endurance, muscle strength, muscle endurance, muscle endurance strength, and power.

2.   Mechanism index: endocrine pressure index of physiological pressure, muscle tissue damage index, muscle tissue energy storage index.

Evaluation Method of Anti-aging Effect of Health Food

Animal models of senescence:

1.   aging rats or mice

2.   long-term experiment using young rats or mice

3.   senescence accelerate mouse (SAM)

4.   drug-induced aging animal:

      (1)   D-galactose induced senescence model

      (2)   Parkinson’s disease animal model (PD)

      (3)   Alzheimer’s disease animal model (AD)

Select an appropriate animal model of senescence for further experiment.

Test items are different depending on the animal model:

1.   Bioactive index of brain aging

2.   Bioactive index of liver, cardiovascular, blood, or kidney aging

3.   Anti-oxidant index

Evaluation Method of Body Fat Accumulation Reducing Effect of Health Food

Male SD rats

1.   High energy diet-induced obesity model (induction in advance)

2.   High energy diet-induced obesity model (induction and treatment at the same time)

Body weight, fat percentage, serum TC, free fatty acid, LDL-C, HDL-C, liver lipids, blood glucose, liver function index, kidney function index, etc.

Evaluation Method of Allergy Constitution Modulating Effect of Health Food

Balb/c or B6 mice

1.   Allergic model induced by intraperitoneal injection of allergen

2.   Respiratory allergy model

3.   allergic rhinitis model

serum antibody analysis, Immune cell proliferation ability, cytokine analysis, pulmonary function measurement or other allergy-related index, natural killer cell activity, phagocytic cell activity, etc.

Evaluation Method of Blood Pressure Regulating Effect of Health Food

Adults over 20 years old who have been diagnosed with higher blood pressure or mild hypertension by a physician and are not taking medication to control blood pressure

Double-blind, randomized, parallel-controlled

Blood pressure, safety monitoring