OECD402

SD rats

Evaluating the lethal dosage and LD50 of the chemicals through dermal route to intake into the body

8 weeks

OECD 403

Wistar rats

Evaluating the lethal dosage and LD50 of the chemicals through inhalation route to intake into the body and observing the effects on lung and/or organs

4 months

OECD 407

SD rats

The chemicals was fed orally for 28 day through three different (low, medium and high) dosage. To evaluate the change in the organs and toxicity after repeated feeding.

clinical symptoms, incidence, mortality, fundoscopy, body weight, feed consumption, feed utilization, hematology, serum biochemistry and electrolytes, urology, organ weight, macroscopic and pathological observations, etc. Finally, the results will present as the no observed adverse effect level, NOAEL.

7 to 10 months (pre-test was contained)

OECD 408

SD rats

The chemicals was fed orally for 90 day to evaluate the long-term chronic toxicity. A longer feed period will better to understand the damage on specific tissues and organs. The three different (low, medium and high) dosage were used. To evaluate the change in the organs and toxicity after repeated feeding.

clinical symptoms, incidence, mortality, fundoscopy, body weight, feed consumption, feed utilization, hematology, serum biochemistry and electrolytes, urology, organ weight, macroscopic and pathological observations, etc. Finally, the results will present as the no observed adverse effect level, NOAEL.

12 to 14 months (pre-test was contained)